Biomarker: UCHL1

Histology type: endometrial carcinoma

Country: Janpanese

Region: Gunma

Study type: retrospective

Followed up time :

Subgroup 1 name : mRNA Low

Subgroup 1 number: 57

Subgroup 2 name: High

Subgroup 2 number: 100

Conclusion: High Expression of Ubiquitin C-terminal Hydrolase L1 Is Associated With Poor Prognosis in Endometrial Cancer Patients

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : high expression

Expression elevation: The staining results were analyzed based on the immunoreactive score using the following 4-step scale: grade 0, no staining; grade 1, <10% tumor cells with weak staining intensity; grade 2, >10% tumor cells with weak to moderate staining intensity; grade 3, 910% tumor cells with strong staining intensity. The final score was designated as low expression or high expression as follows: grade 0 to 2, low expression; grade 3, high expression. All experiments were performed with a positive control (grade 3) and a negative control (grade 0).

Funtion Uniprot: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin (PubMed:9774100, PubMed:8639624, PubMed:12408865, PubMed:23359680). Also binds to free monoubiquitin and may prevent its degradation in lysosomes (By similarity). The homodimer may have ATP-independent ubiquitin ligase activity (PubMed:12408865)

UniProt Link:

Biological function from UniProt: Ubl conjugation pathway

Molecular function from UniProt:

Tissue specificity from UniProt: Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.

Subcellular UniProt: #Cytoplasm #Endoplasmic reticulum #Membrane

Alternative name from UniProt:

Miscellaneous: Oxidation of Met-1, Met-6, Met-12, Met-124 and Met-179 to methionine sulfoxide, and oxidation of Cys-220 to cysteine sulfonic acid have been observed in brains from Alzheimer disease (AD) and Parkinson disease (PD) patients. In AD, UCHL1 was found to be associated with neurofibrillary tangles. In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.

Catalytic activity: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Gene name from HGNC: UCHL1 (PARK5, PGP9.5, Uch-L1)

HPA link: https://www.proteinatlas.org/ENSG00000154277-UCHL1

Tissue specificity RNA from HPA: Group enriched (brain, pituitary gland)

Tissue expression from HPA: Selective expression in CNS, distal renal tubules, islets of Langerhans and spermatogonia in testis.

Subcellular summary HPA Located in Nucleoplasm, Cytosol (Single cell variability)

Cancer prognostic summary HPA Prognostic marker in endometrial cancer (unfavorable) and urothelial cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000154277-UCHL1/tissue

Pathology endo: https://www.proteinatlas.org/ENSG00000154277-UCHL1/tissue/duodenum

HGNC ID: HGNC:12513

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:12513