Biomarker: RNF144B

Histology type: endometrial carcinoma

Country: UK

Region: Manchester

Followed up time :

Subgroup 1 name : malignant

Subgroup 1 number: 14

Subgroup 2 name: normaL

Subgroup 2 number: 8

Conclusion: Substantiate PIR2/RNF144B as a novel candidate for targeted therapy in endometrial cancer.Pir2/Rnf144b is a potential endometrial cancer biomarker that promotes cell proliferation

Sample type : tissue

Sample method: western blotting/IHC/Immunohistochemistry

Expression pattern : PIR2/RNF144B

Funtion Uniprot: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se

UniProt ID: Q7Z419

UniProt Link: https://www.uniprot.org/uniprotkb/Q7Z419/entry

Biological function from UniProt: #Apoptosis #Ubl conjugation pathway

Molecular function from UniProt:

Tissue specificity from UniProt: Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis.

Subcellular UniProt: #Cytoplasm #Membrane #Mitochondrion

Alternative name from UniProt:

Caution: Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family

Activity regulation: [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine

Recommended name: E3 ubiquitin-protein ligase RNF144B

Gene name from HGNC: RNF144B (bA528A10.3, IBRDC2, P53RFP)

HPA class: Enzymes Metabolic proteins

AlphaFold DB: Q7Z419

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/Q7Z419

HPA link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:21578

Tissue specificity RNA from HPA: Tissue enhanced (skeletal muscle)

Tissue expression from HPA: Cytoplasmic expression in several tissues.

Single cell type specificity Cell type enhanced (Suprabasal keratinocytes, Basal keratinocytes, Langerhans cells)

Immune cell specificity: Immune cell enhanced (non-classical monocyte)

Subcellular summary HPA Located in Nucleoli, Mitochondria (Single cell variability)

Cancer prognostic summary HPA Gene product is not prognostic

Pathology link: https://www.proteinatlas.org/ENSG00000137393-RNF144B/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000137393-RNF144B/pathology/endometrial+cancer

OMIM: 618869

OMIM link2: https://www.omim.org/entry/618869

HGNC ID: HGNC:21578

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:21578