Biomarker: CA125

Histology type: rare type

Country: Japan

Region: Saitama

Followed up time :

Subgroup 1 name : Positive

Subgroup 1 number: 31

Subgroup 2 name: Negative

Subgroup 2 number: 9

Conclusion: Co-expression of mesothelin and CA125 may accurately predict OS in mixed carcinomas. Further studies should examine this relationship.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : co-Expression of Mesothelin ( ≥50% (proportion score 3+) and the entire circumference of the cancer cell membrane is stained (intensity score 2+))and CA125(The percentage of CA125-positive cells is ≥50% (proportion score 3+) and the cytoplasm of cancer cell is highly stained (intensity score 2+))

Expression elevation: The percentage of mesothelin- or CA125-positive cells in carcinoma tissues, was as follows:1+, ≥1% and < 10%; 2+, ≥10%and < 50%; and 3+, ≥50% cells stained in entire carcinoma tissue. Staining intensity score is defined as follows: 1+, incomplete membrane staining and/or faint or barely perceptible cytoplasmic staining detected; and 2+, entire circumference of the cell membrane stained and/or moderate to strong cytoplasmic staining. Additionally, a proportion score of 3+ and/or staining intensity score of 2+ was considered as positive mesothelin or CA125 expression.

Statictics: cutoff<65;cutoff>65

Subgroup 1 age: 17;7

Subgroup 2 age: 7;2

Funtion Uniprot: Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.

UniProt ID: Q8WXI7

UniProt Link: https://www.uniprot.org/uniprotkb/Q8WXI7/entry

Molecular function from UniProt:

Tissue specificity from UniProt: Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.

Subcellular UniProt: #Cell membrane #Membrane #Secreted

Alternative name from UniProt:

Miscellaneous: Antigen that is the basis for a widely used serum assay for the monitoring of patients with ovarian epithelial cancer. Due to lack of sensitivity for stage I disease and lack of specificity, it is of little value in the detection of early ovarian cancer. Due to its similarly elevated levels in some nonmalignant conditions, it is not specific enough to be used for population screening.

Recommended name: Mucin-16

Gene name from HGNC: MUC16 (CA125, FLJ14303)

HPA class: Plasma proteins

AlphaFold DB: SMR Q8WXI7

AlphaFold Link: https://swissmodel.expasy.org/repository/uniprot/Q8WXI7?csm=7D5E9A14F70EB90A

Induction: Up-regulated in ovarian cancer cells.

HPA link: https://www.proteinatlas.org/ENSG00000181143-MUC16

Tissue specificity RNA from HPA: Group enriched (adipose tissue, cervix, fallopian tube, salivary gland)

Tissue expression from HPA: Cytoplasmic and membranous expression in glandular cells of cervix, endometrium, fallopian tube, salivary gland and respiratory epithelium.

Single cell type specificity Cell type enhanced (Respiratory ciliated cells, Glandular and luminal cells, Ionocytes, Club cells, Endometrial ciliated cells, Basal respiratory cells)

Immune cell specificity: Not detected in immune cells

Cancer prognostic summary HPA Gene product is not prognostic

Pathology link: https://www.proteinatlas.org/ENSG00000181143-MUC16/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000181143-MUC16/pathology/endometrial+cancer

OMIM: 606154

OMIM link2: https://www.omim.org/entry/606154

HGNC ID: HGNC:15582

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:15582