Biomarker: CXCR7

Biomarker subtype: mRNA

Clinical application: treatment(theranostic target)

Histology type: endometrial carcinoma

Clinical method: RT-PCR/Western blotting

Expression pattern : expression

Validation in cell line : Ishikawa and HEC-1-A cell line

Validation in cell method : RT-PCR and Western blotting,invasion assay ,small interference RNAs of CXCR4 and CXCR7 fragments synthesized, and transfected , FCM

Description: CXCR7 expected to become two target genes for the treatment of endometrial carcinoma.The expression levels of CXCR7 could be inhibited by RNA interference, reducing the cell proliferation, invasion in Ishikawa and HEC-1-A cells.

Funtion description: The expression levels of CXCR4 and CXCR7 could be inhibited by RNA interference, reducing the cell proliferation, invasion in Ishikawa and HEC-1-A cells. In this study, we also observed that treated with CXCR4 and CXCR7 small interfering RNA (siRNA) arrested cells in S phase. CXCL12/CXCR4 and CXCL12/CXCR7 receptor ligand systems affect the invasion of endometrial carcinoma cell line into Ishikawa and HEC-1-A. CXCR4 and CXCR7 were silenced by RNAi, which can inhibit the invasion of Ishikawa and HEC-1-A cell lines.

Approved symbol: ACKR3

Approved name: atypical chemokine receptor 3

Locus type: gene with protein product

HGNC ID: HGNC:23692

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:23692

Symbol status: Approved

Previous symbols: CMKOR1 CXCR7

Alias symbols: RDC1 GPR159

Subgroup: Chemokine receptors

OMIM: 610376

Omim link: https://www.omim.org/entry/610376

NCBI name: 57007

NCBI link: https://www.ncbi.nlm.nih.gov/gene/57007

Summary: This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas. [provided by RefSeq, Jul 2008]

Expression: Broad expression in placenta (RPKM 50.1), urinary bladder (RPKM 36.9) and 22 other tissues

NCBI gene expression https://www.ncbi.nlm.nih.gov/gene/57007#gene-expression

PDB ID: P25106

PDB link: https://www.ebi.ac.uk/pdbe/entry/search/index/?searchParams=%7B%22q_uniprot%22:%5B%7B%22value%22:%22(P25106)%22,%22condition1%22:%22AND%22,%22condition2%22:%22Contains%22%7D%5D,%22resultState%22:%7B%22tabIndex%22:0,%22paginationIndex%22:1,%22perPage%22:%2210%22,%22sortBy%22:%22Sort%20by%22%7D%7D