Biomarker: LSD1

Histology type: endometrioid endometrial carcinoma

Country: China

Region: Harbin

Followed up time :

Subgroup 1 name : Negative

Subgroup 1 number: 59

Subgroup 2 name: Positive

Subgroup 2 number: 147

Conclusion: Overexpression of LSD1 may contribute to the progression of EEA and may thus serve as a new biomarker to predict the prognosis of EEA.

Sample type : tissue

Sample method: Immunohistochemistry/Real-Time PCR

Expression pattern : Overexpression

Expression elevation: The staining intensity was described by scores between 0 and 3 (0 = no reaction, 1 = low, 2 = moderate, 3 = strong). Accordingly, the number of positive cell nuclei was counted and scored between 0 and 4 (0 = no positive cell nuclei,1 = ≤10% positive cell nuclei, 2 = 10%-50% positive cell nuclei, 3 = 51-80% positive cell nuclei, 4 = ≧80% positive cell nuclei). The product of staining intensity and percentage of positive cell nuclei resulted in a score between 0 and 12. Each sample was categorized by this rating score. Scores of 4 or greater were considered positive.

Statictics: cutoff<60;cutoff>60

Subgroup 1 age: 36;74

Subgroup 2 age: 23;73

Funtion Uniprot: Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context (PubMed:15620353, PubMed:15811342, PubMed:16140033, PubMed:16079794, PubMed:16079795, PubMed:16223729). Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed (PubMed:15620353, PubMed:15811342, PubMed:16079794, PubMed:21300290). Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me (PubMed:15620353, PubMed:20389281, PubMed:21300290, PubMed:23721412). May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity (PubMed:16140033, PubMed:16079794, PubMed:16885027, PubMed:21300290, PubMed:23721412). Also acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in AR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A (PubMed:16079795). Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7 (PubMed:20389281).

UniProt ID: O60341

UniProt Link: https://www.uniprot.org/uniprotkb/O60341/entry

Biological function from UniProt: #Transcription #Transcription regulation

Molecular function from UniProt:

Tissue specificity from UniProt: Ubiquitously expressed.

Subcellular UniProt: #Nucleus

Alternative name from UniProt:

Caution: Was previously reported to interact with ASXL1. However, this publication has been retracted.

Catalytic activity: 2 A + 2 H2O + N6,N6-dimethyl-L-lysyl4-[histone H3] = 2 AH2 + 2 formaldehyde + L-lysyl4-[histone H3]

Activity regulation: The N-terminal sequences of INSM1 and SNAI1 compete with histone H3 for the same binding site and thereby inhibit histone demethylation (in vitro).

Recommended name: Lysine-specific histone demethylase 1A

Gene name from HGNC: KDM1A (AOF2, BHC110, KDM1, KIAA0601, LSD1)

HPA class: Disease related genes Enzymes Human disease related genes Potential drug targets

AlphaFold DB: O60341

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/O60341

Induction: Down-regulated during neural differentiation in neuroblastoma cancer.

HPA link: https://www.proteinatlas.org/ENSG00000004487-KDM1A

Tissue specificity RNA from HPA: Low tissue specificity

Tissue expression from HPA: Ubiquitous nuclear expression.

Single cell type specificity Cell type enhanced (Spermatocytes)

Immune cell specificity: Low immune cell specificity

Subcellular summary HPA Located in Nucleoplasm, Cytosol

Cancer prognostic summary HPA Prognostic marker in liver cancer (unfavorable) and colorectal cancer (favorable)

Pathology link: https://www.proteinatlas.org/ENSG00000004487-KDM1A/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000004487-KDM1A/pathology/endometrial+cancer

Phenotype ID: 616728

Disease: Cleft palate, psychomotor retardation, and distinctive facial features (CPRF)

Note1: The disease is caused by variants affecting the gene represented in this entry

OMIM link1: https://www.omim.org/entry/616728

HGNC ID: HGNC:29079

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:29079